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MasterPage
<asp:Button ID="Button1" runat="server" Text="頂層" />
<asp:contentplaceholder id="ContentPlaceHolder1" runat="server">
</asp:contentplaceholder>  

MasterPage2是MasterPage的子母版頁
<asp:Button ID="Button1" runat="server" Text="第二層" />
<asp:contentplaceholder id="ContentPlaceHolder1" runat="server">
</asp:contentplaceholder>  

test.aspx使用MasterPage2
var masterpage = Master as MasterPage; //第二層MasterPage2
var masterpage2 = masterpage.Master as MasterPage;//頂層MasterPage
var content = masterpage2.FindControl("ContentPlaceHolder1") as ContentPlaceHolder;
           
if (content != null)
{
    //第二層(即上一層)需要通過頂層來查找ContentPlaceHolder1，然后找控件
    Button bt2 = content.FindControl("Button2") as Button;
    if (bt2 != null)
    {
        string test = bt2.Text;
    }

}

//頂層可以直接找到
Button bt = masterpage2.FindControl("Button1") as Button;
if (bt != null)
{
    string test = bt.Text;
}